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Expression of multiple ATP receptor subtypes during the differentiation and inflammatory activation of myeloid leukocytes
Author(s) -
Dubyak George R.,
Clifford Erin E.,
Humphreys Benjamin D.,
Kertesy Sylvia B.,
Martin Kathleen A.
Publication year - 1996
Publication title -
drug development research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.582
H-Index - 60
eISSN - 1098-2299
pISSN - 0272-4391
DOI - 10.1002/(sici)1098-2299(199611/12)39:3/4<269::aid-ddr6>3.0.co;2-p
Subject(s) - receptor , myeloid , microbiology and biotechnology , biology , progenitor cell , paracrine signalling , autocrine signalling , receptor expression , p2y receptor , myelopoiesis , proinflammatory cytokine , immunology , inflammation , stem cell , purinergic receptor , biochemistry
ATP can activate both G‐protein‐coupled signaling systems or ATP‐gated channels by stimulating either P2Y‐family or P2X‐family receptors. Our studies indicate that different P2Y‐ and P2X‐family receptors are expressed in mature human phagocytic leukocytes and in myeloid progenitor cells. The P2Y 1 receptor is functionally expressed only in early myeloid progenitor cells, whereas the P 2U /P2Y 2 receptor is expressed in late‐stage progenitor cells and in mature monocytes and neutrophils. The P2X 1 receptor is not expressed in early myeloid progenitors, but it is abundantly expressed when these cells commit to monocytic differentiation. However, the expression of P2X 1 and P 2U /P2Y 2 receptors is significantly down‐regulated when myeloid leukocytes are activated with proinflammatory cytokines. In contrast, these same inflammatory stimuli induce a large up‐regulation of P2Z/P2X 7 receptor expression. We propose that the differential expression of ATP receptor subtypes in various myeloid leukocyte populations indicates that these cells utilize locally released ATP and particular ATP receptors for distinct types of autocrine and paracrine communication. Drug Dev. Res. 39:269–278, 1996. © 1997 Wiley‐Liss, Inc.