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Characterization of new transgenic Big Blue® mouse and rat primary fibroblast cell strains for use in molecular toxicology studies
Author(s) -
Erexson Gregory L.,
Watson David E.,
Tindall Kenneth R.
Publication year - 1999
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1999)34:2/3<90::aid-em6>3.0.co;2-w
Subject(s) - biology , sister chromatid exchange , mutagen , microbiology and biotechnology , fibroblast , micronucleus test , genetics , transgene , genetically modified mouse , mutation , ploidy , gene , cell culture , carcinogen , chemistry , toxicity , dna , organic chemistry
We have established and characterized primary mouse and rat cell strains for studies designed to complement in vivo gene mutation assays using the Big Blue® mouse or rat. Primary fibroblast cell strains, designated BBM1 and BBR1, were derived from a transgenic male Big Blue® B6C3F1 mouse and from a male Big Blue® Fischer‐344 rat, respectively. Both BBM1 and BBR1 are genetically stable and mostly diploid. Both cell strains have low spontaneous frequencies of mutation at the lacI and cII loci as well as low frequencies of sister chromatid exchange and micronuclei formation. In addition, N‐ethyl‐N‐nitrosourea (ENU) induces mutations at the cII locus in both BBM1 and BBR1 cells. These new primary Big Blue® mouse (BBM1) and rat (BBR1) fibroblast cell strains represent useful new models for molecular toxicology studies. Environ. Mol. Mutagen. 34:90–96, 1999 Published 1999 Wiley‐Liss, Inc.