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Quantitative correlation between radiation‐induced mutagenesis in endogenous genes and transgenes of mouse spermatogonial stem cells
Author(s) -
Martus H. J.,
Novak M.,
Blecher D.,
van Duyn–Goedhart A.,
Suter W.,
Gossen J. A.,
van Buul P. P. W.
Publication year - 1999
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1999)34:2/3<216::aid-em22>3.0.co;2-3
Subject(s) - mutagenesis , biology , gene , endogeny , transgene , genetics , mutation , biochemistry
In order to evaluate the pUR288‐plasmid transgenic mouse model, utilizing the bacterial lacZ gene as the mutational target, radiation‐induced mutagenesis was primarily analyzed in spermatogonial stem cells. A combined hydroxyurea (HU)–X‐ray treatment protocol was used, known to sensitize dramatically the induction of mutations in endogenous genes. In the testes of untreated animals, a mutant frequency of 6.7 ± 4.4 × 10 –5 was found. In animals treated with HU or X ray alone, moderate elevations were seen (factors of about 4 and 2 over untreated animal values). In testes of mice having received the HU + X‐ray combination treatment, a mutant frequency of 63.0 ± 36.1 × 10 –5 was found. The results obtained showed a good quantitative correlation between endogenous genes and the transgene, indicating the suitability of pUR288 transgenic mice for also efficiently recording radiation‐induced genetic damage. Radiosensitization, seen in spermatogonial stem cells, was not observed in other studied organs such as spleen, brain, or lung. Environ. Mol. Mutagen. 34:216–220, 1999 © 1999 Wiley‐Liss, Inc.