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Detection of chromosome loss and gain induced by griseofulvin, estramustine, and vanadate in binucleated lymphocytes using FISH analysis
Author(s) -
Migliore Lucia,
ZottiMartelli Laura,
Scarpato Roberto
Publication year - 1999
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1999)34:1<64::aid-em11>3.0.co;2-3
Subject(s) - aneuploidy , biology , chromosome , micronucleus test , microbiology and biotechnology , genetics , griseofulvin , chromatid , marker chromosome , nondisjunction , mitosis , karyotype , chemistry , toxicity , pathology , gene , medicine , organic chemistry
Cytochalasin B‐blocked binucleated human lymphocytes from a healthy male donor were used to detect micronucleus induction and other aneuploidy events (chromosome loss and gain) after treatment with griseofulvin (GF), estramustine (EM), and sodium orthovanadate (Na 3 VO 4 ). A two‐color FISH was performed by using centromeric probes for chromosome 2 (FITC labeled) and the X chromosome (TRITC labeled) to measure chromosome loss and gain events in binucleated cells. GF induced mainly aneuploid binucleates involving the X chromosome, but this was not associated with preferential loss of one of the two chromosomes. EM preferentially induced aneuploidy of chromosome 2, and Na 3 VO 4 of the X chromosome. Our results indicate that chromosome malsegregation events (chromosome loss and/or gain) are probably not randomly induced, suggesting that different mechanisms leading to aneuploidy may be either chromosome‐dependent or compound‐ and dose‐ related. Environ. Mol. Mutagen. 34:64–68, 1999 © 1999 Wiley‐Liss, Inc.