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Formation of DNA adducts in tissues of mouse progeny through transplacental contamination and/or lactation after administration of a single dose of ochratoxin A to the pregnant mother
Author(s) -
PetkovaBocharova T.,
Stoichev I.I.,
Chernozemsky I.N.,
Castegnaro M.,
PfohlLeszkowicz A.
Publication year - 1998
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1998)32:2<155::aid-em11>3.0.co;2-o
Subject(s) - transplacental , carcinogen , biology , offspring , nephrotoxicity , ochratoxin a , mutagen , dna adduct , kidney , mycotoxin , fetus , aristolochic acid , pregnancy , physiology , endocrinology , genetics , microbiology and biotechnology , placenta
Ochratoxin A (OTA) is a mycotoxin which has been detected in foods of plant origin, in edible animal tissues, and in human sera, urine, and milk in many countries. OTA is nephrotoxic and carcinogenic in mice and rats and is suspected to play a key role in the etiology of Balkan endemic nephropathy and/or associated urinary tract tumors. In the present study, some early signs of genetic impairment, including the presence of DNA adducts in target tissues from the progeny of mice after administration of a single OTA dose during late pregnancy, have been investigated. By the 32 P‐postlabeling method, several characteristic DNA adducts with the same R f values were detected in kidney and liver of both the OTA‐treated mice and their progeny—the fetus and the offspring. No adduct was found in tissues from control animals. Different adducts were most important in kidney and liver DNA and some were organ‐specific. High levels of DNA adducts were detected in the kidneys of male progeny, whereas in the female progeny and the mothers they were detected almost exclusively in the liver. This result correlates well with the carcinogenicity in mice: the target organ for males is the kidney, while for females it is the liver. High levels of DNA adducts were also found in fetuses. These results provide evidence for a direct genotoxic action of OTA in the progeny through transplacental contamination, which constitutes a new serious health hazard of exposure to this toxin. Environ. Mol. Mutagen. 32:155–162, 1998 © 1998 Wiley‐Liss, Inc.