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Cytogenetic damage and induction of pro‐oxidant state in human lymphocytes exposed in vitro to gliphosate, vinclozolin, atrazine, and DPX‐E9636
Author(s) -
Lioi Maria B.,
Scarfi Maria R.,
Santoro Antonietta,
Barbieri Rocchina,
Zeni Olga,
Salvemini Francesca,
Di Berardino Dino,
Ursini Matilde V.
Publication year - 1998
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1998)32:1<39::aid-em5>3.0.co;2-6
Subject(s) - vinclozolin , atrazine , genotoxicity , mitotic index , sister chromatid exchange , lymphocyte , biology , sister chromatids , pesticide , chemistry , toxicology , toxicity , genetics , in vitro , mitosis , chromosome , agronomy , organic chemistry , gene
We analyzed chromosome aberrations (CAs), sister chromatid exchanges (SCEs), mitotic index (MI), and glucose 6‐phosphate dehydrogenase (G6PD) enzyme activity in human peripheral lymphocytes from three healthy donors exposed in vitro to different concentrations of gliphosate, vinclozolin, atrazine, and DPX‐E9636. The pesticides gliphosate, vinclozolin, and atrazine have been studied in a broad range of genetic tests with predominantly conflicting or negative results, whereas little is known about the genotoxicity of DPX‐E9636. In our experimental conditions, each chemical compound tested produced a dose‐related increase in the percent of aberrant cells and an increase of SCE/cell. Furthermore, at the highest concentrations of vinclozolin, atrazine, and DPX‐E9636, we observed a significant reduction of the mitotic index. The increase of G6PD activity in exposed lymphocyte cultures strongly indicated an induction of a pro‐oxidant state of the cells as an initial response to pesticide exposure. Environ. Mol. Mutagen. 32:39–46, 1998 © 1998 Wiley‐Liss, Inc.