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Comparison of the types of mutations induced by 7,12‐dimethylbenz[ a ]anthracene in the lacI and hprt genes of Big Blue® rats
Author(s) -
Mittelstaedt Roberta A.,
Manjanatha Mugimane G.,
Shelton Sharon D.,
LynCook Lascelles E.,
Chen James B.,
Aidoo Anane,
Casciano Daniel A.,
Heflich Robert H.
Publication year - 1998
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1998)31:2<149::aid-em6>3.0.co;2-h
Subject(s) - mutation , transversion , transgene , gene , biology , genetics , mutagenesis , microbiology and biotechnology , gene mutation , 7,12 dimethylbenz[a]anthracene , endogeny , point mutation , hypoxanthine guanine phosphoribosyltransferase , reporter gene , dmba , gene expression , carcinogenesis , biochemistry , mutant
An important question regarding the use of transgenic reporter genes to detect mutation in rodents is how the types of mutations recovered in transgenes compare with the types of mutations found in the endogenous genes. In this study, we examined mutations induced by 7,12‐dimethylbenz‐[ a ]anthracene in the lacI transgene and the endogenous hprt gene of lymphocytes from Big Blue® rats and in the hprt gene of lymphocytes from non‐transgenic Fischer 344 rats. The overall mutation profiles found in these genes were remarkably similar: the majority of mutations were base pair substitutions, with the most common mutation being A:T → T:A transversion. Differences were found for the mutational profiles endogenous gene and transgene with respect to the location of the mutations and the orientation of basepair substitutions in the DNA strands. In most cases, these differences could be explained by the nature of the target genes. The results support the use of the lacI transgene for detecting in vivo mutation. Environ. Mol. Mutagen. 31:149–156, 1998 Published 1998 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.