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UVB‐induced mutagenesis in hairless λ lac Z‐transgenic mice
Author(s) -
Frijhoff Anita F. W.,
Rebel Heggert,
Mientjes Edwin J.,
Kelders Marco C. J. M.,
Steenwinkel MarieJosé S. T.,
Baan Robert A.,
Van Zeeland Albert A.,
Roza Len
Publication year - 1997
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1997)29:2<136::aid-em4>3.0.co;2-c
Subject(s) - hairless , mutagenesis , transgene , genetically modified mouse , genetics , biology , mutagen , mutation , lac operon , gene , dna , gene expression
UVB‐induced mutagenesis was studied in hairless 40.6 transgenic mice (MutaMouse), which contain the %lambda;gt10 lacZ shuttle vector as a target for mutagenesis. Mice were exposed at the dorsal side to either single doses of 200, 500, 800, or 1000 J/m 2 UVB or to two successive irradiations of either 200 and 800 J/m 2 UVB, with intervals of 1, 3, or 5 days, or to 800 and 200 J/m 2 UVB with a 5‐day interval. At 23 days after the last exposure, lacZ mutant frequencies (MF) were determined in the epidermis. The lacZ MF increased linearly with increasing dose of UVB. The mutagenic effect of two succes‐sive irradiations appeared to be additive. The UV‐induced mutation spectrum was dominated by G:C b3 A:T transitions at dipyrimidine sites. DNA‐sequence analysis of spontaneously mutated phages showed a diverse spectrum consisting of insertions, deletions and G:C b3 A:T transitions at CpG sites. The results indicate that the hairless %lambda; lacZ ‐transgenic mouse is a suitable in vivo model for studying UVB‐induced mutations. Environ. Mol. Mutagen. 29:136–142, 1997. © 1997 Wiley‐Liss, Inc.