Premium
Plant‐activation of the bicyclic aromatic amines benzidine and 4‐aminobiphenyl
Author(s) -
Ju Young H.,
Plewa Michael J.
Publication year - 1997
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1997)29:1<81::aid-em11>3.0.co;2-d
Subject(s) - benzidine , mutagen , chemistry , ames test , biochemistry , frameshift mutation , carcinogen , stereochemistry , biology , mutation , bacteria , salmonella , genetics , gene
Benzidine and 4‐aminobiphenyl (4‐ABP) are promutagenic bicyclic aromatic amines that are activated into frameshift and base pair substitution mutagens by plant systems. Using the plant cell/microbe coincubation assay, plant‐activated benzidine from 0 to 50 μM induced a concentration‐response in Salmonella typhimurium . At concentrations above 5 μM, plant‐activated benzidine induced frameshift and base pair substitution mutations in the N ‐ or O ‐acetyltransferase over‐expressing strains, DJ460, YG1024, and YG1029. With plant‐activated 4‐ABP, concentrations above 250 μM induced a significant mutagenic response in strains YG1024 and YG1029. A tobacco cell‐free mixture, TX1MX, activated benzidine and 4‐ABP into mutagenic metabolites in S. typhimurium strains YG1024, YG1029, and DJ460. The mutagenic sensitivities of plant‐activated benzidine and 4‐ABP were the same with two different types of plant activation systems, TX1 suspension cells and TX1MX cell‐free medium. The plant activation of these aromatic amines is mediated by tobacco cell peroxidase. Plant‐activated benzidine and 4‐ABP are converted into intermediates that serve as substrates for bacterial or humanacetylCoA: N ‐hydroxyarylamine N ‐acetyl‐transferase to generate the ultimate mutagenic products. Environ. Mol. Mutagen. 29:81–90, 1997 © 1997 Wiley‐Liss, Inc.