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Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice
Author(s) -
Leach Eric G.,
Narayanan Latha,
Havre Pamela A.,
Gunther Edward J.,
Yeasky Toni M.,
Glazer Peter M.
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)28:4<459::aid-em23>3.0.co;2-d
Subject(s) - mutagenesis , point mutation , biology , transgene , mutation , shuttle vector , mutation frequency , genetically modified mouse , microbiology and biotechnology , genetics , mutant , gene , reporter gene , vector (molecular biology) , gene expression , recombinant dna
Transgenic mice carrying multiple copies of a recoverable λ phage shuttle vector carrying the supF mutation reporter gene (λsupF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 × 10 −5 ), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver ( P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue‐specific differences in genetic instability. © 1996 Wiley‐Liss, Inc.