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Mutational specificity: An efficient laboratory protocol for the sequencing of large numbers of lacl mutants recovered from Big Blue® transgenic animals
Author(s) -
Erfle Heather L.,
Walsh David F.,
Holcroft James,
Hague Naheed,
de Boer Johan G.,
Glickman Barry W.
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)28:4<393::aid-em13>3.0.co;2-a
Subject(s) - biology , genetics , transgene , mutant , dna sequencing , mutation , computational biology , microbiology and biotechnology , gene
Mutational spectra provide a powerful approach to investigate both the mutagenic potential and the mechanism of action of suspected mutagens and carcinogens. Recently, transgenic techniques have made it possible to generate mutational spectra in animals. Such a spectrum may consist of 50 to 200 mutants depending on the nature of the mutations, and many spectra can be generated depending on the design of the experiment. This report describes a practical approach for the processing and sequencing of large numbers of lacl mutants recovered from Big Blue® animals. © 1996 Wiley‐Liss, Inc.