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System Issues: Organ variation in the mutagenicity of ethylnitrosourea in Muta™ Mouse: Results of the collaborative study on the transgenic mutation assay by JEMS/MMS
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)28:4<363::aid-em10>3.0.co;2-d
Subject(s) - ethylnitrosourea , mutagenesis , ethyl methanesulfonate , biology , mutagen , genetics , mutation , nitrosourea , genetically modified mouse , mutant , microbiology and biotechnology , transgene , gene , carcinogen , chemotherapy
A collaborative study of the transgenic mouse mutation assay was performed by a subgroup of the Mammalian Mutagenesis Study Group (MMS), which is a suborganization of the Environmental Mutagen Society of Japan (JEMS). Twenty‐six laboratories participated in this collaboration, and ethyl‐nitrosourea (ENU) mutagenesis was studied in eight organs of lacZ transgenic mice (Muta™ Mouse)—liver, spleen, bone marrow, brain, lung, kidney, urinary bladder, and heart. Mice were treated by a single intraperitoneal (ip) injection of 150 mg/kg ENU, and the lacZ mutant frequency (MF) was analyzed by positive selection after 3‐ and 14‐day expression times. The MF in the control group was similar for all organs, approximately 40–60 × 10 −6 . ENU increased MF in all organs except the brain with the highest values (more than 10 times the control value) observed in bone marrow on days 3 and 14, and in spleen on day 14. The MF in urinary bladder increased over 400 × 10 −6 and MFs in liver and lung were more than 150 × 10 −6 on day 14, although no increases were apparent in these organs on day 3. Approximately a doubling of control values was observed in kidney and heart but these were less than 100 × 10 −6 . These results demonstrated that ENU induces organ‐specific mutagenesis with specific expression periods. © 1996 Wiley‐Liss, Inc.