Premium
Comparison of three power supplies used for the single‐cell gel assay
Author(s) -
Vrzoc Maria,
Petras Michael
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)28:2<154::aid-em12>3.0.co;2-e
Subject(s) - methyl methanesulfonate , ethyl methanesulfonate , sensitivity (control systems) , power (physics) , dna , microbiology and biotechnology , chromatography , chemistry , dna damage , toxicology , biology , biochemistry , mutation , physics , engineering , quantum mechanics , electronic engineering , gene
Abstract The alkaline single‐cell gel (SCG) assay, which determines DNA damage in mice treated with 100 mg/kg methyl methanesulfonate (MMS), was run by using three power supplies from Bio‐Rad (models 3000Xi, 200/2.0, and Power Pac 300). Comparisons of the results obtained from the use of these power supplies showed differences in mean DNA tail length to width ratios and profiles of these ratios in individual cells. Model 200/2.0 power supply appeared to enhance significantly the sensitivity of the assay. In purchasing power supplies, there is a need to evaluate their effect on the sensitivity of the test. © 1996 Wiley‐Liss, Inc.