Premium
5‐Azacytidine‐induced 6‐thioguanine resistance at the gpt locus in AS52 cells: Cellular response
Author(s) -
Spencer Diane L.,
Caspary William J.,
Hines Kimberly C.,
Tindall Kenneth R.
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)28:2<100::aid-em5>3.0.co;2-j
Subject(s) - mutagen , cytotoxicity , biology , mutant , apoptosis , population , programmed cell death , doubling time , cell , microbiology and biotechnology , cell culture , azacitidine , genetics , in vitro , carcinogen , gene , medicine , gene expression , dna methylation , environmental health
Treatment of AS52 cells with 5‐azacytidine resulted in an induction of 6‐thioguanine‐resistant (6TG′) colonies, which reached a maximum by an expression time of 9 days. Dose responses for both cytotoxicity and mutation induction were determined following treatment with 5‐azacytidine. At 20 μM treatment, 5‐azacytidine exposure resulted in about 50% survival. Mutant frequency reached a maximum at 10 μM. At concentrations between 10 and 20 μM, 5‐azacytidine was a potent mutagen but did not exhibit a dose response. Although many compounds both induce cell death and affect the growth rate of cells, 5‐azacytidine specifically induced cell death and did not affect the doubling time of the surviving treated cell population. © 1996 Wiley‐Liss, Inc.