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Detection of centromeres in vinblastine‐ and radiation‐induced micronuclei of human lymphocytes using FISH with an α satellite pancentromeric DNA probe
Author(s) -
Huber R.,
Salassidis K.,
Kulka U.,
Braselmann H.,
Bauchinger M.
Publication year - 1996
Publication title -
environmental and molecular mutagenesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1
H-Index - 87
eISSN - 1098-2280
pISSN - 0893-6692
DOI - 10.1002/(sici)1098-2280(1996)27:2<105::aid-em5>3.0.co;2-f
Subject(s) - micronucleus test , vinblastine , centromere , biology , micronucleus , microbiology and biotechnology , clastogen , satellite dna , fluorescence in situ hybridization , genetics , chemistry , chromosome , gene , toxicity , chemotherapy , organic chemistry
Fluorescence in situ hybridisation (FISH) with a human alphoid satellite pancentromeric DNA probe was used to detect centromeres in micronuclei of human lymphocytes induced by γ‐irradiation and by Vinblastine sulfate. In a cytokinesis‐block micronucleus assay a dose‐dependent increase of micronuclei was detected for both agents. 72–89% of Vinblastine‐induced micronuclei, but only 7–48% of radiation‐induced micronuclei showed centromere‐positive fluorescence signals. Vinblastine treatment frequencies of centromere‐negative micronuclei did not increase compared to control values, nor did frequencies of centromere‐positive micronuclei in irradiated lymphocytes. Since FISH with an α satellite DNA probe allows the direct detection of centromeric DNA sequences the spindle damaging or clastogenic effectiveness of a compound can be easily and reliably examined in a cytokinesis‐block micronucleus assay in human lymphocytes. © 1996 Wiley‐Liss, Inc.