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A functional investigation of tumor suppressor gene activities in a nasopharyngeal carcinoma cell line HONE1 using a monochromosome transfer approach
Author(s) -
Cheng Yue,
Stanbridge Eric J.,
Kong Heidi,
Bengtsson Ulla,
Lerman Michael I.,
Lung Maria Li
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(200005)28:1<82::aid-gcc10>3.0.co;2-8
Subject(s) - nasopharyngeal carcinoma , biology , chromosome , cdkn2a , suppressor , cancer research , microbiology and biotechnology , carcinogenesis , tumor suppressor gene , cell culture , gene , genetics , medicine , radiation therapy
Abstract Monochromosome transfers of selected chromosomes into a nasopharyngeal carcinoma (NPC) cell line were performed to determine if tumor suppressing activity for NPC mapped to chromosomes 9, 11, and 17. Current information from cytogenetic and molecular allelotyping studies indicate that these chromosomes may harbor potential tumor suppressor genes vital to NPC. The present results show the importance of CDKN 2 A on chromosome 9 in NPC development. There was no functional suppression of tumor development in nude mice with microcell hybrids harboring the newly transferred chromosome 9 containing an interstitial deletion at 9p21, whereas transfection of CDKN 2 A into the NPC HONE1 cells resulted in obvious growth suppression. Whereas intact chromosome 17 transfers into HONE1 cells showed no functional suppression of tumor formation, chromosome 11 was able to do so. Molecular analysis of chromosome 11 tumor segregants indicated that at least two tumor suppressive regions mapping to 11q13 and 11q22–23 may be critical for the development of NPC. Genes Chromosomes Cancer 28:82–91, 2000. © 2000 Wiley‐Liss, Inc.