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Identification of a 1‐Mb common region at 16q24.1–24.2 deleted in hepatocellular carcinoma
Author(s) -
Bando Koichi,
Nagai Hisaki,
Matsumoto Satoshi,
Koyama Masaaki,
Kawamura Naoki,
Tajiri Takashi,
Onda Masahiko,
Emi Mitsuru
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(200005)28:1<38::aid-gcc5>3.0.co;2-a
Subject(s) - hepatocellular carcinoma , contig , biology , microsatellite , allele , hccs , phenotype , gene , pathology , cancer research , genetics , medicine , genome
To identify the location of one or more putative tumor suppressor genes that may be involved in hepatocellular carcinoma (HCC), we examined 96 such tumors for their patterns of allelic loss at 21 microsatellite marker loci distributed along chromosome arm 16q. Allelic loss at one or more loci was observed in 58 (60%) of these tumors. Detailed deletion mapping identified a distinct commonly deleted region located within an interval flanked by D 16 S 534 and D 16 S 3091 at 16q24.1–24.2. By constructing a physical map consisting of a YAC contig across the region, the extent of the deleted region was determined to be less than 1 Mb. Among the tumors for which clinical data were available, allelic loss at 16q24.1–24.2 was more frequent in tumors arising from liver cirrhosis compared to HCCs arising from chronic hepatitis (30/42, 71%, vs. 13/33, 39%; P = 0.0054). Additionally, allelic loss at 16q24.1–24.2 was frequently observed in small tumors and early‐stage tumors as well as in tumors of more advanced phenotype. Genes Chromosomes Cancer 28:38–44, 2000. © 2000 Wiley‐Liss, Inc.