Premium
Isolation and characterization of a novel TP53‐inducible gene, TP53TG5, which suppresses growth and shows cell cycle‐dependent transition of expression
Author(s) -
Isaka Shigeyuki,
Takei Yoshiki,
Tokino Takashi,
Koyama Kumiko,
Miyoshi Yasuo,
Suzuki Mikio,
Takahashi Eiichi,
Azuma Chihiro,
Murata Yuji,
Nakamura Yusuke
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(200004)27:4<345::aid-gcc2>3.0.co;2-3
Subject(s) - biology , gene , plasmid , transfection , cell cycle , microbiology and biotechnology , dna , homology (biology) , chromosome , cell culture , genetics
Abstract Through a strategy of direct cloning of TP53‐binding DNA sequences from human genome DNA, we have identified a novel TP53‐target gene, termed TP53TG5 (TP53‐target gene 5). This gene, localized to chromosome band 20q13.1 by fluorescence in situ hybridization, encodes a 290‐amino‐acid peptide with no significant homology with any known proteins in the public database. A colony‐formation assay using human glioblastoma cell line T98G, which lacks wild‐type TP53 and expresses no endogenous TP53TG5, revealed a growth‐suppressive effect of the TP53TG5 gene product. Furthermore, immunohistochemical studies, following transfection of T98G with plasmid designed to express green fluorescent protein‐fused TP53TG5, revealed cell cycle‐dependent intracellular localization of this protein. Our results suggest that functional studies of TP53TG5 may provide new insights into the complex physiological activities of TP53. Genes Chromosomes Cancer 27:345–352, 2000. © 2000 Wiley‐Liss, Inc.