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A 3‐cM commonly deleted region in 6q21 in leukemias and lymphomas delineated by fluorescence in situ hybridization
Author(s) -
Zhang Yanming,
Matthiesen Peter,
Harder Svetlana,
Siebert Reiner,
Castoldi Gianluigi,
Calasanz Maria Jose,
Wong Kit Fai,
Rosenwald Andreas,
Ott German,
Atkin Niels B.,
Schlegelberger Brigitte
Publication year - 2000
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(200001)27:1<52::aid-gcc7>3.0.co;2-x
Subject(s) - breakpoint , fluorescence in situ hybridization , biology , lymphoma , chromosome , gene , leukemia , in situ hybridization , tumor suppressor gene , cytogenetics , microbiology and biotechnology , genetics , cancer research , carcinogenesis , immunology , gene expression
Deletions of the long arm of chromosome 6 (6q) are frequent chromosome aberrations in non‐Hodgkin lymphomas (NHLs) and acute lymphoblastic leukemias (ALLs). It is presumed that one or more tumor suppressor genes are localized on 6q. By means of fluorescence in situ hybridization (FISH), we attempted to detect and delineate deletions of 6q in leukemias and lymphomas. We performed FISH on 148 cases of lymphoma and acute leukemia using a panel of 36 YAC probes distributed from 6q12 to 6q27 and a centromeric probe of chromosome 6 as internal control. Deletions of 6q that included a 7‐cM commonly deleted region in 6q21 were detected in 59 patients who had B‐ and T‐cell low‐grade and high‐grade NHL and ALL. FISH with two YAC probes flanking this region was performed on an additional 97 cases of NHL and leukemia. Deletions in 6q21 were detected in an additional 21 cases. In five cases of high‐grade B‐ and T‐cell NHL and ALL, the deletion breakpoints were located within the commonly deleted region. To define the deletion breakpoints exactly and to narrow this region further, FISH was performed with six additional YAC probes that have been physically localized within this region. A 3‐cM (4–5 Mb) commonly deleted region in 6q21 was delineated. Our study suggests that this commonly deleted region harbors a putative tumor suppressor gene involved in the pathogenesis of both low‐grade and high‐grade NHL and ALL. Genes Chromosomes Cancer 27:52–58, 2000. © 2000 Wiley‐Liss, Inc.