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Cytogenetic clonality analysis of megakaryocytes in myelodysplastic syndrome by dual‐color fluorescence in situ hybridization and confocal laser scanning microscopy
Author(s) -
Lom Kirsten van,
Houtsmuller Adriaan B.,
van Putten Wim L.J.,
Slater Rosalyn M.,
Löwenberg Bob
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199908)25:4<332::aid-gcc4>3.0.co;2-4
Subject(s) - in situ , fluorescence in situ hybridization , confocal , confocal laser scanning microscopy , in situ hybridization , confocal microscopy , fluorescence , microscopy , pathology , laser scanning , fluorescence microscope , laser , materials science , biology , optics , chemistry , medicine , biophysics , microbiology and biotechnology , genetics , chromosome , physics , gene , gene expression , organic chemistry
In the myelodysplastic syndrome (MDS), cytogenetic abnormalities are often present and can be used as markers in studies for cell lineage involvement. Little is known of the involvement of the megakaryocytic lineage due to the variable ploidy of these cells. We applied dual‐color fluorescence in situ hybridization (FISH) to routinely prepared bone marrow (BM) smears of cytogenetically normal patients and seven patients with MDS and monosomy 7 or trisomy 8. Probes specific for the centromeric regions of chromosomes 7 and 8 were detected with fluorescein isothiocyanate (FITC) and Texas Red, respectively. This enabled us to assess the ratio between the numbers of chromosomes 7 and 8 in the polyploid cells. We utilized confocal laser scanning microscopy to count the FITC and Texas Red FISH signals in the different focal layers of the megakaryocytes. Fifty‐six megakaryocytes in six normal BM smears were analyzed, giving a mean ratio of 1.0, a standard deviation (SD) of 0.12, and a range of 0.8–1.33. This ratio was applied to evaluation of clonal involvement of individual megakaryocytes in the patients with MDS. In two patients with monosomy 7, the majority of the megakaryocytes were monosomic. In the five patients with trisomy 8, all or a majority of the analyzed megakaryocytes were trisomic. These results add direct evidence that in MDS megakaryocytes are involved in the malignant clone. Genes Chromosomes Cancer 25:332–338, 1999. © 1999 Wiley‐Liss, Inc.