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Myeloid‐ and lymphoid‐specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia
Author(s) -
Coignet Lionel J.A.,
Lima Carmen S.P.,
Min Toon,
Streubel Berthold,
Swansbury John,
Telford Nick,
Swanton Soheila,
Bowen Angela,
Nagai Masami,
Catovsky Daniel,
Fonatsch Christa,
Dyer Martin J.S.
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199907)25:3<222::aid-gcc4>3.0.co;2-c
Subject(s) - chromosomal translocation , biology , myeloid leukemia , cancer research , retinoblastoma , leukemia , acute myeloblastic leukemia , myeloid , chromosome , breakpoint , genetics , microbiology and biotechnology , gene
Abnormalities of chromosome band 13q14 occur in hematologic malignancies of all lineages and at all stages of differentiation. Unlike other chromosomal translocations, which are usually specific for a given lineage, the chromosomal translocation t(12;13)(p12;q14) has been observed in both B‐cell and T‐cell precursor acute lymphoblastic leukemia (BCP‐, TCP‐ALL), in differentiated and undifferentiated acute myeloblastic leukemia (AML), and in chronic myeloid leukemia (CML) at progression to blast crisis. The nature of these translocations and their pathologic consequences remain unknown. To begin to define the gene(s) involved on chromosome 13, we have performed fluorescence in situ hybridization (FISH) using a panel of YACs from the region, on a series of 10 cases of acute leukemia with t(12;13)(p12;q14) and 1 case each with “variant” translocations including t(12;13)(q21;q14), t(10;13)(q24;q14) and t(9;13)(p21;q14). In 8/13 cases/cell lines, the 13q14 break fell within a single 1.4 Mb CEPH MegaYAC. This YAC fell immediately telomeric of the forkhead ( FKHR ) gene, which is disrupted in the t(2;13)(q35;q14) seen in pediatric alveolar rhabdomyosarcoma. Seven of the 8 cases with breaks in this YAC were AML. In 4/13 cases, the 13q14 break fell within a 1.7‐Mb YAC located about 3 Mb telomeric of the retinoblastoma ( RB1 ) gene: all 4 cases were ALL. One case of myelodysplastic syndrome exhibited a break within 13q12, adjacent to the BRCA2 gene. These data indicate the presence of myeloid‐ and lymphoid‐specific breakpoint cluster regions within chromosome band 13q14 in acute leukemia. Genes Chromosomes Cancer 25:222–229, 1999. © 1999 Wiley‐Liss, Inc.