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Low frequency of rearrangements of the homeobox gene HOXA9 /t(7;11) in adult acute myeloid leukemia
Author(s) -
Kwong YokLam,
Pang Annie
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199905)25:1<70::aid-gcc11>3.0.co;2-e
Subject(s) - myeloid leukemia , homeobox , medicine , leukemia , cancer research , biology , genetics , gene , transcription factor
Translocation (7;11)(p15;p15) is uncommon in acute myeloid leukemia (AML) and may have a predilection to occur in Asian patients. It results in a reciprocal fusion of the homeobox gene HOXA9 and the nucleoporin gene NUP98 . To ascertain the molecular features and the clinicopathological and prognostic significance of t(7;11)(p15;p15), 208 adult Chinese patients with AML were screened by Southern blot analysis with an HOXA9 cDNA probe and reverse transcription–polymerase chain reaction for NUP98/HOXA9 . Three cases were found to have rearrangement of the HOXA9 gene. Two cases were found to have an NUP98/HOXA9 fusion transcript, with a breakpoint at NUP98 different from that previously described. The remaining case had no rearrangement of NUP98 , nor was NUP98/HOXA9 detected. All positive cases had refractory AML with poor treatment outcome. In conclusion, t(7;11)/ HOXA9 rearrangement was a rare event (3/208, 1.5%) in AML, even in a population where it was considered to be more prevalent. Two breakpoints in NUP98 may occur, and this is of importance in the design of primers to amplify t(7;11). Genes Chromosomes Cancer 25:70–74, 1999. © 1999 Wiley‐Liss, Inc.