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Gonadoblastoma, mixed germ cell tumor, and Y chromosomal genotype: Molecular analysis in four patients
Author(s) -
Muroya Koji,
Ishii Tomohiro,
Nakahori Yutaka,
Asakura Yumi,
Tachibana Katsuhiko,
Masuno Mitsuo,
Imaizumi Kiyoshi,
Tanaka Yukichi,
Kawada Yasusada,
Yukizane Shigenori,
Ogata Tsutomu
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199905)25:1<40::aid-gcc6>3.0.co;2-8
Subject(s) - gonadoblastoma , biology , germ cell , microbiology and biotechnology , germ cell tumors , locus (genetics) , y chromosome , genetics , chromosome , cancer research , gene , karyotype , chemotherapy
This study reports on Y chromosomal genotypes of three patients with gonadoblastoma and one patient with gonadoblastoma and mixed germ cell tumor. Molecular analysis for 35 Y chromosomal loci was performed for DNA samples taken from peripheral leukocytes and lymphoblastoid cell lines, showing that the four patients shared the region between DYS267 at interval 4A and DYF50S1 at interval 6D, with the exception of the region around DYS202 at interval 5K. In the patient with gonadoblastoma and mixed germ cell tumor, Y chromosomal material was preserved in the gonadoblastoma but was lost from the mixed germ cell tumor. The results, in conjunction with previous reports, suggest that GBY (gonadoblastoma locus on the Y chromosome) may be located to a roughly 5‐Mb pericentromeric region between DYS267 at interval 4A and DYS270 at interval 5A. The presence of Y chromosomal material in gonadoblastoma is consistent with GBY being involved in the development of gonadoblastoma, and the absence of Y chromosomal material in mixed germ cell tumor would be explained as a consequence of Y chromosomal loss from rapidly proliferating gonadal cancer cells. Genes Chromosomes Cancer 25:40–45, 1999. © 1999 Wiley‐Liss, Inc.