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Molecular cytogenetic delineation of the breakpoint at 18q21.1 in low‐grade B‐cell lymphoma of mucosa‐associated lymphoid tissue
Author(s) -
Akagi Tomoaki,
Tamura Akiko,
Motegi Mutsuhito,
Suzuki Ritsuro,
Hosokawa Yoshitaka,
Nakamura Shigeo,
Morishima Yasuo,
Seto Masao,
Taniwaki Masafumi
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199904)24:4<315::aid-gcc4>3.0.co;2-4
Subject(s) - breakpoint , clone (java method) , biology , malt lymphoma , chromosomal translocation , contig , lymphoma , mucosa associated lymphoid tissue , microbiology and biotechnology , bacterial artificial chromosome , locus (genetics) , chromosome , comparative genomic hybridization , genetics , gene , genome , immunology
Extranodal malignant non‐Hodgkin lymphoma of mucosa‐associated lymphoid tissue type (MALT lymphoma) represents a subtype of B‐cell lymphoid malignancies with distinct clinicopathological features and is often associated with a favorable prognosis. Recent cytogenetic studies have revealed that t(11;18)(q21;q21) is a characteristic chromosomal aberration in low‐grade B‐cell MALT‐type lymphoma. In the present study, we employed florescence in situ hybridization analysis using contiguous YAC clones mapped to the 18q21.1 region to identify a YAC clone, y789F3, encompassing the breakpoint of t(11;18)(q21;q21) in a MALT lymphoma. P1 artificial chromosome (PAC) contigs constructed on this YAC clone were used to analyze the breakpoint region. PAC clone 264m4 was observed on normal chromosome 18 and on der(18), and PAC clone 879n10 on normal chromosome 18 and on der(11), confirming that the breakpoint is located between these two PAC clones. We also found that a region of approximately 500 kb between the two PAC clones was deleted. These results indicate that the locus between PAC clones 264m4 and 879n10 at 18q21.1 involved in t(11;18) translocation or associated deletion plays an important role in the development of MALT lymphoma. Genes Chromosomes Cancer 24:315–321, 1999. © 1999 Wiley‐Liss, Inc.