Premium
TP53 gene mutations in plasma DNA of cancer patients
Author(s) -
Silva Jose M.,
Gonzalez Rocio,
Dominguez Gemma,
Garcia Jose M.,
España Pilar,
Bonilla Felix
Publication year - 1999
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199902)24:2<160::aid-gcc10>3.0.co;2-c
Subject(s) - gene , cancer , exon , dna , biology , dna sequencing , lung cancer , microbiology and biotechnology , mutation , gene mutation , breast cancer , cancer research , genetics , medicine , pathology
Using different molecular techniques, DNA has been shown to be present in plasma of patients with several types of tumors. Mutations of TP53 are the most common genetic changes in human cancers. We investigated the presence of TP53 gene mutations in plasma DNA of breast and small cell lung cancer patients. Tumor and plasma DNA of 25 patients were studied by PCR‐SSCP and direct sequencing, through exons 5, 6, 7, and 8, of TP53 . Six cases of mutations in tumor DNA were observed that, in 3 cases (50%), were also identified in plasma of the same patients. Mutations of the TP53 gene are seen in plasma DNA of cancer patients, and may prove to be a useful new tool in the management of these patients. Genes Chromosomes Cancer 24:160–161, 1999. © 1999 Wiley‐Liss, Inc.