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Chromosomal constitution of human spermatocytic seminomas: Comparative genomic hybridization supported by conventional and interphase cytogenetics
Author(s) -
Rosenberg Carla,
Mostert Marijke C.,
Schut Tom Bakker,
van de Pol Mirjam,
van Echten Jannie,
de Jong Bauke,
Raap Anton K.,
Tanke Hans,
Oosterhuis J. Wolter,
Looijenga Leendert H. J.
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199812)23:4<286::aid-gcc2>3.0.co;2-6
Subject(s) - comparative genomic hybridization , cytogenetics , biology , ploidy , karyotype , chromosome , genetics , aneuploidy , interphase , gene
No data on the chromosomal constitution of spermatocytic seminomas are available thus far because of their rarity. Ploidy analysis performed on paraffin‐embedded cases showed varying results from (near‐) diploid to aneuploid. We applied comparative genomic hybridization on four snap‐frozen primary spermatocytic seminomas of three different patients. Conventional cytogenetic analysis was successful in one, and “interphase cytogenetics” with centromeric region‐specific probes was applied to another. The results from comparative genomic hybridization showed almost exclusively numerical chromosomal aberrations, in agreement with the data from karyotyping. Despite the limited number of cases studied, a nonrandom pattern of chromosome imbalances was detected: chromosome 9 was gained in all spermatocytic seminomas. This suggests that that this aberration plays a role in the development of this cancer. Interphase cytogenetics shows that the copy number of most chromosomes ranges from two to four, with an average of near trisomic. This constitutes the first report on the chromosomal constitution of spermatocytic seminomas. Genes Chromosomes Cancer 23:286–291, 1998. © 1998 Wiley‐Liss, Inc.