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Identification of a 100‐kb region of common allelic loss on chromosome bands 10q25–q26 in human endometrial cancer
Author(s) -
Yamakawa Hiromitsu,
Nagase Satoru,
Yuki Michihiro,
Shiwaku Hiromi O.,
Furukawa Toru,
Yoshinaga Kousuke,
Soeda Eiichi,
Hoshi Masato,
Hayashi Yutaka,
Sato Shinji,
Yajima Akira,
Horii Akira
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199809)23:1<74::aid-gcc12>3.0.co;2-e
Subject(s) - cosmid , contig , biology , genetics , xhoi , chromosome , fluorescence in situ hybridization , restriction map , clone (java method) , gene mapping , microbiology and biotechnology , gene , restriction enzyme , genome , nucleic acid sequence , bamhi
In human endometrial cancer, we have previously identified a 790‐kb region of common allelic loss in chromosome bands 10q25–q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two‐color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which Not I, Sal I, Sma I, and Xho I were clustered, suggesting the possible existence of a CpG island. Genes Chromosomes Cancer 23:74–77, 1998. © 1998 Wiley‐Liss, Inc.