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A common region of allelic loss on chromosome region 3p25.3–26.3 in nasopharyngeal carcinoma
Author(s) -
Deng Longwen,
Jing Ning,
Tan Guoling,
Zhou Ming,
Zhan Fenghuang,
Xie Yi,
Cao Li,
Li Guiyuan
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199809)23:1<21::aid-gcc4>3.0.co;2-8
Subject(s) - loss of heterozygosity , nasopharyngeal carcinoma , microsatellite , biology , allele , genetics , chromosome , carcinogenesis , chromosome 3 , positional cloning , chromosomal region , tumor suppressor gene , deletion mapping , gene , locus (genetics) , medicine , radiation therapy
Loss of heterozygosity (LOH) of chromosome arm 3p has been commonly observed in carcinomas of various tissues, including those of nasopharyngeal carcinoma (NPC). To determine the frequency and extent of allelic loss in NPC, we investigated 16 loci on chromosome bands 3p21–26 in 24 tumor tissues by microsatellite analysis. LOH on 3p21–26 was found in 16 of 24 (66.7%) tumors. The highest frequency of allelic loss was found in two adjacent loci, D3S1620 (11/22, 50%) and D3S1560 (9/18, 50%). Eight cases showed LOH in one contiguous region and 5 cases in more than one region. Samples 1, 3, 4, 7, 8, 10, 16, 17, 18, 19, and 22 had a contiguous stretch of allelic loss between D3S1297 and D3S1597. The smallest common LOH/deletion region seems likely to lie between D3S1297 (3p26.3–26.2) and D3S1560 (3p25.3). The allelic loss map defined here will facilitate finer mapping of putative tumor suppressor gene loci and positional cloning of such genes, which may play a role in carcinogenesis of NPC. Genes Chromosomes Cancer 23:21–25, 1998. © 1998 Wiley‐Liss, Inc.