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Isolation of a novel TP53 target gene from a colon cancer cell line carrying a highly regulated wild‐type TP53 expression system
Author(s) -
Takei Yoshiki,
Ishikawa Shinji,
Tokino Takashi,
Muto Tetsuichiro,
Nakamura Yusuke
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199809)23:1<1::aid-gcc1>3.0.co;2-y
Subject(s) - gene , biology , isolation (microbiology) , cell culture , gene expression , colorectal cancer , wild type , genetics , cancer research , cancer , microbiology and biotechnology , bioinformatics , mutant
We established a colon cancer cell line SW480‐LOWTP53‐1 carrying a wild‐type TP53 transgene that is inducible under control of the lactose operon. Induction of this transgene by isopropyl‐β‐D‐thiogalactoside (IPTG) arrests growth of the transfected cells. To investigate cellular responses related to the TP53 signaling pathway to induce growth arrest, we applied a differential display method to screen mRNAs isolated from this cell line and looked for genes whose expression was activated or suppressed after induction of wild‐type TP53. Subsequent Northern blot analysis confirmed that expression of one novel gene was regulated by wild‐type TP53. The cDNA, termed TP53TG1 (TP53 target gene 1), contained an open reading frame of 270 nucleotides encoding 90 amino acids. Under conditions of cellular stress (ultraviolet irradiation or exposure to bleomycin or cisplatin), expression of TP53TG1 was induced in a wild‐type TP53‐dependent manner, indicating that this gene is likely to play an important role in the signaling pathway of TP53 and may function in response to cellular damage. Genes Chromosomes Cancer 23:1–9, 1998. © 1998 Wiley‐Liss, Inc.