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FISH characterization of head and neck carcinomas reveals that amplification of band 11q13 is associated with deletion of distal 11q
Author(s) -
Jin Yuesheng,
Höglund Mattias,
Jin Charlotte,
Martins Carmo,
Wennerberg Johan,
Åkervall Jan,
Mandahl Nils,
Mitelman Felix,
Mertens Fredrik
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199808)22:4<312::aid-gcc7>3.0.co;2-y
Subject(s) - biology , amplicon , fluorescence in situ hybridization , locus (genetics) , metaphase , microbiology and biotechnology , breakpoint , karyotype , gene duplication , gene , chromosome , genetics , polymerase chain reaction
In order to characterize homogeneously staining regions (HSR) and other 11q13 rearrangements identified cytogenetically, we performed fluorescence in situ hybridization (FISH) using a CCND1 cosmid and five YAC clones spanning chromosomal bands 11q13–14 on metaphase cells from 14 primary and one metastatic head and neck carcinomas. At the cytogenetic level, a total of 17 HSR were detected in ten cases: five were in derivative chromosomes 11 in band 11q13, and 12 were located in other derivative chromosomes. Other forms of 11q13 rearrangements were observed in five cases, whereas two cases had normal chromosomes 11. FISH analysis demonstrated that all HSR but two were derived from the 11q13 band. The size of the amplicon varied from case to case, but the amplification always included the region covered by YAC 55G7, which contains the CCND1 locus. The amplification of CCND1 was confirmed by use of a CCND1 cosmid. We also showed that most of the cases (9 of 11) with 11q13 amplification had lost material from distal 11q. The breakpoints were mapped by FISH and were shown to cluster to the region between YACs 55G7 and 749G2. We conclude that loss of gene(s) in distal 11q may be as important as amplification of genes in 11q13 for the biological aggressiveness of head and neck carcinomas. Genes Chromosomes Cancer 22:312–320, 1998. © 1998 Wiley‐Liss, Inc.