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Isolation and characterization of a novel human pancreas‐specific gene, pancpin , that is down‐regulated in pancreatic cancer cells
Author(s) -
Ozaki Kouichi,
Nagata Masami,
Suzuki Mikio,
Fujiwara Tsutomu,
Miyoshi Yasuo,
Ishikawa Osamu,
Ohigashi Hiroaki,
Imaoka Shingi,
Takahashi Eiichi,
Nakamura Yusuke
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199807)22:3<179::aid-gcc3>3.0.co;2-t
Subject(s) - pancreatic cancer , pancreas , biology , gene , open reading frame , carcinogenesis , serpin , microbiology and biotechnology , missense mutation , mutation , cancer , serine protease , complementary dna , cancer research , genetics , protease , peptide sequence , biochemistry , enzyme
By means of the differential display method, we isolated a novel human gene that is expressed specifically in pancreas. The cDNA, designated “pancpin,” contained an open reading frame of 1,215 nucleotides encoding a 405 amino acid protein, showing a high degree of similarity to serine protease inhibitors belonging to the serpin superfamily. To investigate its possible role in pancreatic carcinogenesis, we looked for genetic alterations of this gene in pancreatic cancer cell lines and primary pancreatic cancer tissues. Expression of pancpin was barely detectable in any of the four pancreatic cancer cell lines examined, and very weak also in 10 of 13 pancreatic cancer tissues. A somatic missense mutation at codon 221 was found in two of 16 primary pancreatic cancers. These findings indicate that down‐regulation of pancpin expression may play a significant role in development or progression of pancreatic cancer. Genes Chromosomes Cancer 22:179–185, 1998. © 1998 Wiley‐Liss, Inc.