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Overrepresentation of 7q31 and 17q in renal cell carcinomas
Author(s) -
Glukhova Liubov,
Goguel AnneFrançoise,
Chudoba Ilse,
Angevin Eric,
Pavon Christine,
TerrierLacombe MarieJosé,
Meddeb Mounira,
Escudier Bernard,
Bernheim Alain
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199807)22:3<171::aid-gcc2>3.0.co;2-t
Subject(s) - polysomy , comparative genomic hybridization , biology , clone (java method) , cancer research , pathology , cytogenetics , chromosome , cancer , gene , fluorescence in situ hybridization , genetics , medicine
Xenografts from four metastatic renal cell carcinomas (RCCs) were established in immunodeficient mice. All tumors exhibited cytogenetic features specific for the papillary subtype, namely, partial or total polysomy of chromosomes 7 and 17 and integrity of 3p. Cytogenetic analysis of the initial and xenografted tumors indicated that although clonal characteristics were consistently maintained in xenografts derived from metastases, a minor clone had been selected for in the xenografts derived from the primary tumors. Reverse painting and comparative genomic hybridization (CGH) allowed us to localize minimal overrepresented genomic regions to 7q31, where the MET protooncogene is located, and to 17q. Other overrepresented regions were 8q in all xenografts and Xq22–qter in three of them. The gain of genetic material from these regions may be a key factor ensuring the papillary nature of RCCs and their survival in xenografts. Genes Chromosomes Cancer 22:171–178, 1998. © 1998 Wiley‐Liss, Inc.