Premium
Identification of allelic loss on chromosome arm 6p in human astrocytomas by arbitrarily primed polymerase chain reaction
Author(s) -
Saitoh Yoshiki,
Bruner Janet M.,
Levin Victor A.,
Kyritsis Athanassios P.
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199807)22:3<165::aid-gcc1>3.0.co;2-u
Subject(s) - locus (genetics) , biology , polymerase chain reaction , microbiology and biotechnology , southern blot , microsatellite , loss of heterozygosity , gene , allele , inverse polymerase chain reaction , fluorescence in situ hybridization , genetics , genomic dna , chromosome , multiplex polymerase chain reaction
We employed the arbitrarily primed polymerase chain reaction (AP‐PCR), which is a PCR‐based genomic fingerprinting technique, to detect novel genetic alterations in human astrocytomas. DNA fingerprints generated by arbitrary primers were compared in normal lymphocytes and tumor tissues from the same individuals. We cloned and sequenced an AP‐PCR band showing a greatly decreased intensity in tumor tissue DNA, relative to normal. Southern blot showed that this sequence was homozygously deleted in the tumor cell genome. Semiquantitative PCR analysis further showed significant decreases of signals in seven of 24 tumors, consistent with homozygous deletion of this sequence. The deleted sequence was localized to chromosome region 6p21.1 by the fluorescence in situ hybridization method. Microsatellite analysis also showed frequent allelic loss of this locus compared to neighboring loci in astrocytoma tissues. These results suggest the presence of a novel tumor suppressor gene at the 6p21.1 locus. Genes Chromosomes Cancer 22:165–170, 1998. © 1998 Wiley‐Liss, Inc.