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Isolation, mapping, and functional analysis of a novel human cDNA ( BNIP3L ) encoding a protein homologous to human NIP3
Author(s) -
Matsushima Mieko,
Fujiwara Tsutomu,
Takahashi Eiichi,
Minaguchi Takeo,
Eguchi Yutaka,
Tsujimoto Yoshihide,
Suzumori Kaoru,
Nakamura Yusuke
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199803)21:3<230::aid-gcc7>3.0.co;2-0
Subject(s) - complementary dna , chromosome band , gene , biology , homology (biology) , microbiology and biotechnology , open reading frame , homologous chromosome , dna , nucleic acid sequence , genetics , chromosome , peptide sequence , fluorescence in situ hybridization
We have isolated a novel cDNA that encodes a product showing significant sequence homology (56% identity) to human NIP3, a protein thought to interact with adenovirus E1B19kD and human BCL2 proteins. This cDNA contains an open reading frame of 657 nucleotides encoding a 219 amino acid polypeptide. The gene, designated BNIP3L , was expressed in all 16 normal human tissues examined; we mapped it to chromosome band 8p21 by fluorescence in situ hybridization. Introduction of the BNIP3L gene into six different cancer‐cell lines caused significant growth suppression in each of them, while no such effect occurred when the antisense cDNA or the vector DNA was transfected, indicating that BNIP3L may function as a tumor suppressor. Genes Chromosomes Cancer 21:230–235, 1998. © 1998 Wiley‐Liss, Inc.