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Characterization of the breakpoints in a t(8;13)(p11;q12) translocation from a patient with myeloproliferative disease using fluorescence in situ hybridization
Author(s) -
Chernova Olga,
Still Ivan,
Kalaycio Matt,
Hoeltge Gerald,
Cowell John K.
Publication year - 1998
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199802)21:2<160::aid-gcc12>3.0.co;2-v
Subject(s) - breakpoint , chromosomal translocation , yeast artificial chromosome , fluorescence in situ hybridization , biology , chromosome , genetics , cytogenetics , abnormality , karyotype , chromosome 22 , microbiology and biotechnology , gene , gene mapping , medicine , psychiatry
We used fluorescence in situ hybridization to characterize the molecular position of the breakpoints in a t(8;13)(p11;q12) reciprocal translocation from a patient with an atypical myeloproliferative disorder. This structural chromosome abnormality is characteristic of this specific disease and occurs often as the only chromosome abnormality in the malignant cells. Yeast artificial chromosome (YAC) analysis has demonstrated that the 8p11 breakpoint lies within a region defined by YAC 959A4 and that the 13q12 breakpoint is spanned by YAC 769F9. Identifying the position of the breakpoints in this rearrangement provides the means to search for candidate genes rearranged by this highly specific structural chromosome abnormality. Genes Chromosomes Cancer 21:160–165, 1998. © 1998 Wiley‐Liss, Inc.