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Human/mouse microcell hybrid based elimination test reduces the putative tumor suppressor region at 3p21.3 to 1.6 cM
Author(s) -
Szeles Anna,
Yang Ying,
Sandlund Agneta Manneborg,
Kholodnyuk Irina,
Kiss Hajnalka,
KostAlimova Maria,
Zabarovsky Eugene R.,
Stanbridge Eric,
Klein George,
Imreh Stephan
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199712)20:4<329::aid-gcc3>3.0.co;2-3
Subject(s) - microcell , suppressor , cancer research , biology , microbiology and biotechnology , computer science , cancer , genetics , operating system
We have previously identified an approximately 7 cM long common eliminated region (CER), involving the 3p21.3 markers AP20R, D3S966, D3S3559, D3S1029, WI‐7947, D3S2354, AFMb362wb9, and D3S32, in human chromosome 3/A9 mouse fibrosarcoma microcell hybrid (MCH) derived SCID mouse tumors. We now report the results of our more detailed analysis on 24 SCID mouse tumors derived from two MCH lines that originally carried intact human chromosomes 3. They were analyzed by fluorescence in situ hybridization (FISH) painting and PCR, using 24 markers covering the region between D3S1611 and D3S1235 at 3p22‐p21.2. D3S32 and D3S2354 were regularly eliminated during in vivo tumor growth, whereas the other 22 markers, D3S1611, ACAA , D3S1260, WI‐692, AP20R, D3S3521, D3S966, D3S1029, D3S643, WI‐2420, MST1, GNAI2 , D3S1235, D3S1298, GLB1 , WI‐4193, D3S3658, D3S3559, D3S3678, WI‐6400, WI‐7947, and WI‐10865, were regularly retained. We have defined a common eliminated region of approximately 1.6 cM (designated as CER1) inside the 7 cM CER described earlier. CER1 is flanked distally by D3S1029 and proximally by D3S643. Genes Chromosomes Cancer 20:329–336, 1997. © 1997 Wiley‐Liss, Inc.