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Isolation of osteosarcoma‐associated amplified DNA sequences using representational difference analysis
Author(s) -
Simons Annet,
Janssen Irene M.,
Suijkerbuijk Ron F.,
Veth René P.H.,
Pruszczynski Maciej,
Hulsbergenvan de Kaa Christina A.,
du Manoir Stanislas,
van Kessel Ad Geurts
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199710)20:2<196::aid-gcc10>3.0.co;2-p
Subject(s) - representational difference analysis , biology , microbiology and biotechnology , clone (java method) , dna , osteosarcoma , fluorescence in situ hybridization , comparative genomic hybridization , chromosome , genomic dna , gene , genetics , cancer research , gene expression
Comparative genomic hybridization analysis of a primary osteosarcoma and its metastasis revealed two regions of DNA amplification, one at 17p11.2‐12 and one at 19q12‐13. Subsequent representational difference analysis of the primary tumor resulted in the isolation of two distinct tumor‐amplified DNA fragments originating from chromosome 19. A YAC clone corresponding to one of the two isolated DNA fragments was used for fluorescence in situ hybridization on normal human lymphocyte metaphases and tumor‐derived nuclei. This resulted in the localization of this YAC to 19q12‐13.1 and confirmed the amplification status of the isolated fragment in the tumors. The availability of such RDA‐isolated sequences may be instrumental in the search for genes relevant for tumor development. Genes Chromosomes Cancer 20:196–200, 1997. © 1997 Wiley‐Liss, Inc.