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Normal FHIT transcripts in renal cell cancer‐ and lung cancer‐derived cell lines, including a cell line with a homozygous deletion in the FRA3B region
Author(s) -
van den Berg Anke,
Draaijers Tineke G.,
Kok Klaas,
Timmer Tineke,
Van der Veen Anneke Y.,
Veldhuis Patrick M. J. F.,
de Leij Lou,
Gerhartz Claus D.,
Naylor Susan L.,
Smith David I.,
Buys Charles H. C. M.
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199708)19:4<220::aid-gcc3>3.0.co;2-z
Subject(s) - fhit , biology , exon , gene , microbiology and biotechnology , alternative splicing , adenocarcinoma , intron , cell culture , genetics , cancer , gene product , cancer research , tumor suppressor gene , gene expression , carcinogenesis
The recently identified FHIT gene encompasses the FRA3B region and the breakpoint of a constitutive t(3;8) occurring in a family with hereditary renal cell cancer. Occurrence of aberrant transcripts in different types of tumours has led to the suggestion that FHIT might play a critical role in the development of various types of cancer. We have analyzed the gene and its transcripts in lung cancers and renal cell cancer‐derived cell lines. A lung adenocarcinoma cell line, GLC‐A2, appeared to have a homozygous deletion in intron 5 of FHIT. RT‐PCR analysis revealed a normal‐sized PCR product in all of the cell lines, including GLC‐A2. A number of them had an additional aberrant product. Analysis of a great number of control cell lines and tissues showed that the majority of these also had aberrant PCR products in addition to a normal‐sized PCR product. Different specimens of the same cell type showed variable additional RT‐PCR products. Normal‐sized PCR products had a sequence identical to the FHIT sequence. PCR products longer than normal had insertions of different sizes at different positions. With three exceptions, PCR products shorter than normal represented FHIT sequences missing one or more entire exons. Thus, the presence of aberrant transcripts is not cancer‐specific. Conceivably, sequences responsible for the instability of the FRA3B region are being transcribed into FHIT pre‐mRNA and may cause the abnormal splicing and processing of the transcripts. Genes Chromosom. Cancer 19:220–227, 1997. © 1997 Wiley‐Liss, Inc.