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Variable FHIT transcripts in non‐neoplastic tissues
Author(s) -
Panagopoulos Ioannis,
Thelin Sofia,
Mertens Fredrik,
Mitelman Felix,
Åman Pierre
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199708)19:4<215::aid-gcc2>3.0.co;2-#
Subject(s) - variable (mathematics) , biology , computational biology , genetics , mathematics , mathematical analysis
We used nested reverse transcriptase PCR to investigate the expression of the FHIT gene, a presumptive tumor suppressor gene located in chromosomal band 3p14.2, in non‐neoplastic samples. Multiple transcripts of the FHIT gene were found in peripheral blood lymphocytes, skeletal muscle, and liver of healthy individuals, as well as in a cell line derived from synovial tissue. The data indicate that variable splicing of the FHIT transcript, leading to deletions of exons and thus anomalous or absent FHIT protein production, occurs frequently in non‐neoplastic tissues. Hence, the finding of multiple nonfunctional FHIT transcripts is not tumor‐specific and cannot be used as a genetic marker of neoplasia. Genes Chromosom. Cancer 19:215–219, 1997. © 1997 Wiley‐Liss, Inc.