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Abnormalities of chromosomes 3 and 8 in posterior uveal melanoma correlate with prognosis
Author(s) -
Sisley Karen,
Rennie Ian G.,
Parsons M. Andrew,
Jacques Rhona,
Hammond David W.,
Bell Sharon M.,
Potter Anthony M.,
Rees Robert C.
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199705)19:1<22::aid-gcc4>3.0.co;2-2
Subject(s) - monosomy , melanoma , chromosome , uvea , ciliary body , pathology , biology , chromosome 7 (human) , cytogenetics , fluorescence in situ hybridization , chromosome 3 , aneuploidy , karyotype , medicine , cancer research , genetics , gene
Posterior uveal melanomas have nonrandom alterations affecting chromosomes 3, 6, and 8. Loss of chromosome 3 in uveal melanoma has been shown to act as a predictor of disease‐free and overall survival. To confirm the significance of chromosome 3 loss and to extend the observations to include those of the associated alterations of chromosome 8, we have conducted a cytogenetic analysis on a series of 42 tumours from patients with primary uveal melanoma who were followed up for a median of 31 months (range = 8‐96 months). Abnormalities of chromosomes 3 and 8 were the commonest changes and were confirmed in 10 tumours using flourescence in situ hybridization. Monosomy of chromosome 3 was found in 21 (50%) of the tumours, and 23 (54%) tumours had additional copies of 8q. Alterations of chromosomes 3 and 8 were found occurring together in 19 (45%) of the tumours and were significantly associated with a ciliary body component ( P < 0.0001). Prognostic indicators and changes of chromosomes 3 and 8 were analysed for correlation with patient survival. Of the chosen parameters, only ciliary body involvement ( P = 0.003), monosomy of chromosome 3 ( P = 0.0007), and additional copies of 8q ( P = 0.003) correlated with reducted survival. Evaluation of the dosage effect of additional copies of chromosome arm 8q showed a significant association with reduced survival ( P = 0.0001), which was also predictive of a decreased disease‐free interval ( P = 0.01). Thus, the cytogenetic analysis of uveal melanoma may provide a valuable predictor of prognosis. Genes Chromosom. Cancer 19:22–28, 1997. © 1997 Wiley‐Liss, Inc.