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BCL6 can repress transcription from the human immunodeficiency virus type I promoter/enhancer region
Author(s) -
Baron Beverly W.,
Desai Manisha,
Baber L. Julia,
Paras Leilani,
Zhang Qiang,
Sadhu Annamma,
Duguay Stephen,
Nucifora Giuseppina,
McKeithan Timothy W.,
ZeleznikLe Nancy
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199705)19:1<14::aid-gcc3>3.0.co;2-3
Subject(s) - enhancer , transcription (linguistics) , biology , virology , promoter , human immunodeficiency virus (hiv) , genetics , transcription factor , gene , gene expression , philosophy , linguistics
The gene BCL6 encodes a zinc finger protein with similarities to transcription factors. We previously reported that a number of viral genomes, including human immunodeficiency virus type I (HIV‐I), contain sequences which are similar to the BCL6 DNA‐binding consensus in their promoter regions. Electrophoretic mobility shift assays showed that the full‐length BCL6 protein extracted from transferred COS cells and a bacterially expressed truncated protein containing the BCL6 zinc fingers can bind specifically to DNA from the U3 promoter/enhancer region of HIV‐I. Transient transfections were performed to analyzed the effects of the BCL6 protein on luciferase expression driven by the HIV‐I long terminal repeat (LTR) sequences. Full‐length BCL6 significantly repressed luciferase activity compared with multiple controls. We conclude that the BCL6 protein can bind to the HIV‐I promoter‐enhancer region and contains a domain upstream of its zinc fingers that can repress transcription from the HIV‐I LTR. Genes Chromosom. Cancer 19:14–21, 1997. © 1997 Wiley‐Liss, Inc.