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Molecular status of individual CFU‐GM colonies derived from chemotherapy‐mobilised peripheral blood stem cells in chronic myeloid leukaemia
Author(s) -
Broughton Caroline M.,
Sherrington Paul,
Pender Norman T.,
Clark Richard E.
Publication year - 1997
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199704)18:4<292::aid-gcc7>3.0.co;2-w
Subject(s) - cytarabine , haematopoiesis , idarubicin , chemotherapy , biology , progenitor cell , abl , myeloid , stem cell , cd34 , immunology , philadelphia chromosome , colony forming unit , myeloid leukemia , microbiology and biotechnology , cancer research , andrology , medicine , gene , chromosomal translocation , genetics , receptor , tyrosine kinase , bacteria
Following chemotherapy in chronic myeloid leukaemia (CML), some peripheral blood (PB) cells may be Philadelphia (Ph) chromosome negative. The BCR‐ABL mRNA status of residual Ph + progenitors is not known. We examined the BCR‐ABL mRNA status of individual colony‐forming‐unit granulocyte‐macrophage (CFU‐GM) colonies derived from PB harvested following chemotherapy. Seven patients were treated with 200 mg/m 2 /day cytarabine and 20 mg/m 2 /day Idarubicin and followed by Lenograstim. PB collections commenced daily when the white blood cell count reached 0.6 × 10 9 /l and continued until at least 5 × 10 8 /kg nucleated cells were obtained. CD34 + cells, Ph status, and CFU‐GM were estimated at each harvest. For each patient, up to 24 individual CFU‐GM colonies were analysed for BCR‐ABL status. Two cases were BCR‐ABL negative on all colonies and completely Ph − , and another case was BCR‐ABL positive in all colonies and completely Ph + . In contrast, in two patients all colonies were BCR‐ABL negative, despite virtually complete Ph + metaphases. The final assessible case had five of nine BCR‐ABL negative colonies, despite 94% Ph + metaphases. After chemotherapy priming, the PB may contain Ph + CFU‐GM that do not express BCR‐ABL. Genes Chromosom. Cancer 18:292–298, 1997. © 1997 Wiley‐Liss, Inc.