Premium
Identification of a yeast artificial chromosome spanning the 8q12 translocation breakpoint in pleomorphic adenomas with t(3;8)(p21;q12)
Author(s) -
Röijer Eva,
Kas Koen,
Klawitz Ingrid,
Bullerdiek Jörn,
Van de Ven Wim,
Stenman Göran
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199611)17:3<166::aid-gcc4>3.0.co;2-2
Subject(s) - breakpoint , chromosomal translocation , identification (biology) , chromosome , yeast artificial chromosome , genetics , yeast , biology , computational biology , gene , botany , gene mapping
A subgroup of pleomorphic adenomas of the salivary glands is characterized by translocations involving chromosome 8, with consistent breakpoints at 8q12. As part of a positional cloning effort to isolate the gene(s) affected by these translocations we now report the mapping of the 8q12 breakpoint in two primary pleomorphic adenomas with the recurrent t(3;8)(p21;q12). Yeast artificial chromosome (YAC) clones corresponding to eight different loci in 8q11‐12 were isolated and mapped by fluorescence in situ hybridization (FISH). The t(3;8) breakpoint was mapped within a 1 Mb region flanked by MOS proximally and by the genetic marker D8S166 distally. One YAC within this region was shown to span the t(3;8) breakpoint in two tumors. This YAC will provide an excellent tool for isolating the gene(s) at the breakpoint(s) in adenomas with t(3;8). Genes Chromosom Cancer 17:166–171 (1996) . © 1996 Wiley‐Liss, Inc.