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ML‐1 cell line lacks a germline MLL locus
Author(s) -
Strout Matthew P.,
Mrózek Krzysztof,
Hein Kristiina,
Sait Sheila N. J.,
Shows Thomas B.,
Aplan Peter D.
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199607)16:3<204::aid-gcc8>3.0.co;2-2
Subject(s) - biology , allele , locus (genetics) , genetics , germline , breakpoint , chromosomal translocation , microbiology and biotechnology , derivative chromosome , gene , chromosome 22
Gene rearrangements involving MLL (also known as ALLI, HRX, or Htrx ) are among the most common molecular abnormalities associated with acute leukemia. These leukemias generally have one allele involved in a rearrangement, while the remaining allele is uninvolved and demonstrates a germline MLL configuration. In this study, we describe a leukemic cell line that does not have a germline MLL allele and thus cannot produce a normal MLL gene product. We show that the ML‐1 cell line, derived from a patient with acute myeloid leukemia, has one allele involved in a t(6; 11)(q27;q23), while the remaining MLL allele is deleted. Cloning of the genomic breakpoints on the derivative(6) and der(11) chromosomes demonstrated a balanced translocation between MLL on chromosome band 11q23 and AF6 on chromosome band 6q27. Sequence analysis of the derivative chromosomes revealed that a 186‐bp segment of MLL intron 6, downstream of the breakpoint, had been duplicated, inverted, and inserted between MLL and AF6 on the der(11) chromosome. In light of the fact that ML‐1 cells can be induced to differentiate along the granulocyte and macrophage lineages, the finding that ML‐1 lacks a germline MLL allele demonstrates that a normal MLL gene is not required for survival, proliferation, or differentiation of this cell line. Genes Chromosom Cancer 16:204–210 (1996). © 1996 Wiley‐Liss, Inc.