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Mapping of chromosomal gains and losses in primitive neuroectodermal tumors by comparative genomic hybridization
Author(s) -
Schütz Barbara R.,
Scheurlen Wolfram,
Krauss Jürgen,
du Manoir Stanislas,
Joos Stefan,
Bentz Martin,
Lichter Peter
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199607)16:3<196::aid-gcc7>3.0.co;2-u
Subject(s) - comparative genomic hybridization , biology , southern blot , gene duplication , chromosome , polymerase chain reaction , in situ hybridization , cancer research , genetics , dna , microbiology and biotechnology , gene , messenger rna
A series of 18 primitive neuroectodermal tumors (PNETs), the most common malignant central nervous system tumors of childhood, were analyzed with the recently developed approach of comparative genomic hybridization (CGH). In five cases, in which only small amounts of DNA were available, universal polymerase chain reaction was successfully applied to generate adequate probe material. In 15 tumors, chromosomal imbalances were elicited, most frequently involving chromosome 17 (loss of 17p and gain of 17q). Further recurrent imbalances included gains of the distal regions of 4p, 5p, 5q, 7q, 8q, and 9p. High‐level amplifications were found on 2p24 (one case) and 8q24 (three cases), suggesting involvement of the protooncogenes MYCN and MYC, respectively. In one of these cases, Southern blot analysis could be performed, proving high‐copy‐number amplification of MYC. Interestingly, none of the three patients with high‐copy‐number amplifications of ( MYC responded to therapy. Genes Chromosom Cancer 16:196–203 (1996). © 1996 Wiley‐Liss, Inc.