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Loss of the chromosomal region 5q11‐q31 in the myeloid cell line HL‐60: Characterization by comparative genomic hybridization and fluorescence in situ hybridization
Author(s) -
Shipley Janet,
WeberHall Stephen,
Birdsall Sandra
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199603)15:3<182::aid-gcc7>3.0.co;2-z
Subject(s) - fluorescence in situ hybridization , comparative genomic hybridization , in situ hybridization , in situ , biology , genetics , microbiology and biotechnology , genome , chromosome , gene , chemistry , gene expression , organic chemistry
Comparative genomic hybridization was used to identify the regions of genomic gain and loss in the myeloid cell line HL‐60. These included amplification at 8q24 corresponding to previous reports of overrepresentation of the MYC gene; loss of material from the short arms of chromosomes 9 (9p21‐p23), 10, and 17; loss of the chromosome regions 9q32‐qter and 14q11‐q24; and an extra copy of chromosome 18. Additionally, deletion of the 5q11‐q31 region was noted and was associated with translocation of chromosome 5 material to chromosomes 16 and a dic(5;17)(q11;p11) chromosome (previously described as mar3). Loss of chromosome 5 material in myeloid malignancies, including the M2 subtype from which HL‐60 was derived, is usually associated with interstitial deletions of the long arm, including the critical 5q31 region, resulting in a 5q‐chromosome. The HL‐60 cell line may be a useful model to investigate the role of potential tumour suppressor genes associated with loss of 5q material in myeloid leukaemias. Genes Chromosom Cancer 15:182–186 (1996) . © 1996 Wiley‐Liss, Inc.