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Fusion of an ETS ‐family gene, EIAF , to EWS by t(17;22)(q12;q12) chromosome translocation in an undifferentiated sarcoma of infancy
Author(s) -
Kaneko Yasuhiko,
Yoshida Koichi,
Handa Masafumi,
Toyoda Yasunori,
Nishihira Hirokazu,
Tanaka Yukichi,
Sasaki Yoshiroh,
Ishida Setsuko,
Higashino Fumihiro,
Fujinaga Kei
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199602)15:2<115::aid-gcc6>3.0.co;2-6
Subject(s) - fusion gene , biology , fusion protein , southern blot , chromosomal translocation , gene , complementary dna , gene family , microbiology and biotechnology , sarcoma , chromosome , enhancer , breakpoint , gene expression , genetics , recombinant dna , pathology , medicine
EIAF is a newly isolated ETS ‐family gene that is located on 17q21 and codes for the adenovirus EIA enhancer‐binding protein. In our chromosome analysis of 18 of the Ewing family of tumors and undifferentiated sarcomas, we found t(17;22)(q12;q12) in an MIC2 antigen‐positive undifferentiated sarcoma of infancy. On Southern blot analysis, EWS and EIAF cDNA probes hybridized to the same rearranged band, indicating that an EWS‐EIAF fusion gene was formed in the tumor. Further Southern blot analysis using four EIAF cDNA probes of different sizes showed that the breakpoint lies in the region upstream to the ETS domain of the EIAF gene. EIAF may be the fourth ETS ‐family gene to be identified forming a fusion gene with EWS. We assume that the RNA binding domain of EWS may have been replaced by the DNA binding domain of EIAF in the EWS‐EIAF fusion protein as in other fusion proteins previously characterized in Ewing sarcoma and other types of sarcomas. Genes Chromosom Cancer 15:115–121 (1996). © 1996 Wiley‐Liss, Inc.