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Chromosome microdissection in leukemia: A powerful tool for the analysis of complex chromosomal rearrangements
Author(s) -
Jonveaux Philippe,
Le Coniat Maryvonne,
Derre Josette,
Flexor MarieAnge,
Daniel MarieThérèse,
Berger Roland
Publication year - 1996
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/(sici)1098-2264(199601)15:1<26::aid-gcc4>3.0.co;2-6
Subject(s) - microdissection , biology , chromosomal translocation , chromosome , fluorescence in situ hybridization , cytogenetics , marker chromosome , karyotype , metaphase , microbiology and biotechnology , genetics , chromosome 15 , gene
In many human cancers the presence of marker chromosomes or unbalanced translocations prevents complete karyotypic analysis. Chromosome microdissection has become an increasingly important method for assessing chromosome rearrangements. However, most studies using chromosome microdissection have been carried out on established cancer cell lines that provide an unlimited supply of abnormal metaphase cells. We have routinely performed microdissection of as few as three marker chromosome copies from short‐term cultures of acute myeloid leukemias, followed by in vitro DNA amplification and fluorescence in situ hybridization (FISH) to normal metaphase spreads. Using this type of “reverse chromosome painting,” we were able to characterize precisely the chromosomal constitution of each marker chromosome in the samples, confirming the diagnostic usefulness of microdissection in cancer cytogenetics. In addition, in one leukemia with atypical cytological features, microdissection enabled us to detect a novel retinoic acid receptor α gene rearrangement. Genes Chromosom Cancer 15:26–33 (1996) . © 1996 Wiley‐Liss, Inc.