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Expression of the murine complement regulatory protein Crry by glial cells and neurons
Author(s) -
Davoust Nathalie,
Nataf Serge,
Holers V. Michael,
Barnum Scott R.
Publication year - 1999
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(199908)27:2<162::aid-glia6>3.0.co;2-k
Subject(s) - biology , complement (music) , neuroscience , microbiology and biotechnology , gene , genetics , phenotype , complementation
The expression of the murine complement regulatory protein, Crry, in the CNS remains largely unexplored. In this study, we examined murine astrocytes and microglia purified from neonatal brain and sections of adult murine brain for the expression of Crry. Using RT‐PCR, immunohistochemistry, in situ hybridization, flow cytometry, and Western blot analysis, we demonstrated that astrocytes and microglia express Crry protein and RNA. Crry expression is greater on microglia than astrocytes and, as determined by Western blot analysis, each cell type expresses a Crry protein of different molecular weight. Interestingly, neuronal expression of Crry was seen only at the RNA level. These data demonstrate Crry expression by astrocytes, microglia, and neurons in the murine CNS and suggest that Crry may play an important role in protecting the CNS against complement‐mediated damage. GLIA 27:162–170, 1999. © 1999 Wiley‐Liss, Inc.