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Substratum of pleiotrophin (HB‐GAM) stimulates rat CG‐4 line oligodendrocytes to adopt a bipolar morphology and disperse: Primary O‐2A progenitor glial cells disperse similarly on pleiotrophin
Author(s) -
Rumsby Martin,
Suggitt Faye,
Haynes Laurie,
Hughson Elaine,
Kidd Dan,
McNulty Shaun
Publication year - 1999
Publication title -
glia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.954
H-Index - 164
eISSN - 1098-1136
pISSN - 0894-1491
DOI - 10.1002/(sici)1098-1136(199906)26:4<361::aid-glia10>3.0.co;2-q
Subject(s) - pleiotrophin , progenitor cell , biology , neurite , oligodendrocyte , neuroglia , microbiology and biotechnology , cell culture , stem cell , in vitro , neuroscience , biochemistry , central nervous system , growth factor , genetics , myelin , receptor
Pleiotrophin (HB‐GAM), an extracellular matrix‐associated protein with a high content of basic amino acid residues, is expressed in the central nervous system during late pre‐ and early post‐natal development and promotes neurite outgrowth in vitro. Here, we show that, on a substratum of pleiotrophin formed from a 5 or 10 μg/ml solution, undifferentiated rat CG‐4 line oligodendrocytes adopt a bipolar morphology and disperse over the substratum, as we have previously shown with poly‐L‐lysine (Rumsby et al. Neurosci Res Commun 23:101–109, 1998). On pleiotrophin substrata formed from coating solutions of 1μg/ml and below, CG‐4 line cells form aggregates and do not disperse, as is also the case with poly‐L‐lysine. The same dispersing effect is observed with rat primary 0–2A progenitor glial cells on pleiotrophin substrata from solutions of 5 and 10μg/ml: 0–2A cells aggregate together on pleiotrophin substrata formed from lower concentrations and do not disperse. A pleiotrophin substratum enhances proliferation of CG‐4 line oligodendrocytes and primary 0–2A progenitor glial cells. The results show that pleiotrophin provides a substratum that can influence progenitor oligodendrocyte morphology, aid cell dispersion, and perhaps also enhance progenitor oligodendrocyte cell growth. GLIA 26:361–367, 1999. © 1999 Wiley‐Liss, Inc.